Journal: Journal of Advanced Research

Article Title: OGT regulates histone demethylation and alleviates osteoarthritis progression by O-GlcNAcylation of KDM6B

doi: 10.1016/j.jare.2026.01.003

Figure Lengend Snippet: OGT is downregulated in cartilage from OA patients. (A) Differentially expressed genes in cartilage from control individuals and OA patients. (B) Volcano plot showing upregulated and downregulated genes in OA cartilage. (C) Relative expression levels of OGT in cartilage from control and OA patients. (D) GSEA demonstrating protein O-linked glycosylation enriched in OA cartilage (NES = -1.53). (E) Representative X-ray and cartilage histology images from OA patients. (F, G) Western blot analysis and quantification of OGT in intact and damaged cartilage (n = 6). (H) Representative H&E and Safranin O/Fast Green (SO&FG) staining of intact and damaged cartilage (n = 3. Scale bars, 1000 μm). (I) Representative immunofluorescence images of OGT in intact and damaged cartilage (n = 3. Scale bars, 100 μm). (J) Representative H&E and SO&FG staining of human cartilage treated with IL-1β (10 ng/mL) (n = 3. Scale bars, 500 μm). (K) Representative immunofluorescence images of OGT in explants treated with IL-1β (10 ng/mL) (n = 3. Scale bars, 100 μm). (L, M) Quantification of positive cells in panels G and H (n = 3). Data are presented as the means ± SEM. P values are from two-tailed unpaired Student’s t -test. *P < 0.05, **P < 0.01, and ***P < 0.001.

Article Snippet: The cartilage was cut into small pieces, washed three times with sterile phosphate-buffered saline (PBS), and cultured in DMEM/F-12 medium supplemented with 10 % FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin for 24 h. Subsequently, the explants were cultured for an additional 7 days in medium supplemented with 10 ng/mL recombinant human IL-1β (Abclonal, RP00002).

Techniques: Control, Expressing, Glycoproteomics, Western Blot, Staining, Immunofluorescence, Two Tailed Test

Journal: iScience

Article Title: Periostin drives psoriasis progression through the stimulation of cutaneous nerve fibers

doi: 10.1016/j.isci.2026.116021

Figure Lengend Snippet: Periostin expression in psoriatic skin parallel increased neural innervation (A) tSNE plot of full thickness mouse skin including both epidermis and skin stroma ( GSE129218 ). (B) Marker gene expression for each cell type, where dot size and color represent the percentage of marker gene expression (pct. exp) and the averaged scaled expression (avg. exp. scale) value, respectively. (C) RNA expression of Postn for each cell type, where dot size and color represent the percentage of marker gene (pct. exp) and the averaged scaled expression (avg. exp. scale) value, respectively. (D and E) GO analysis of significantly differentially expressed genes in fibroblast and keratinocyte clusters revealed enrichment of axon growth and neuro-related signaling pathways. (F) Relative mRNA expression levels of Periostin in normal and Psoriasis skin, n = 6. (G) Representative immunofluorescent co-staining of POSTN and DAPI from Vehicle or IMQ-treated mouse skin sections and quantification, n = 8. (H) Representative immunofluorescent co-staining of POSTN, Tuj1, and DAPI from skin of normal or patients with psoriasis, the dotted line separates the epidermis and the dermis layers, n = 3, scale bars, left: 50 μm, right: 100 μm. (I) Western blotting of POSTN protein levels in whole skin lysates from Vehicle or IMQ-induced psoriasis-like mouse model, n = 3, and its quantification (J). Quantitative data in (F, G, and J) are presented as mean ± SEM. ns , not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Two-tailed unpaired Student’s t test in (F, G, and J).

Article Snippet: For recombinant periostin stimulation, 1 μg/mL recombinant POSTN (rPOSTN; ABclonal) was added to the culture medium, which was refreshed daily throughout the experiment.

Techniques: Expressing, Marker, Gene Expression, RNA Expression, Protein-Protein interactions, Staining, Western Blot, Two Tailed Test

Journal: iScience

Article Title: Periostin drives psoriasis progression through the stimulation of cutaneous nerve fibers

doi: 10.1016/j.isci.2026.116021

Figure Lengend Snippet: Periostin deficiency alleviated psoriasis-like lesions and reduced skin hyperkeratosis in mice (A) Schematic diagram of the experimental procedure for establishing the psoriasis mouse model. (B) Representative skin images were generated from WT and Postn −/− mice with or without IMQ administration. (C) Changes in skin thickness during psoriasis model establishment. (D) Skin scaling score during psoriasis model establishment. (E) HE staining of normal or psoriasis-like skin in WT and Postn −/− mice. Scale bars, left 3 pictures: 50 μm, right: 100 μm. (F) Quantification of skin acanthosis of IMQ-induced mice, n = 6. (G) Immunofluorescence staining to detect skin keratinocyte marker CK14 and quantification of CK14 + cells in the epidermis of IMQ-induced WT or Postn −/− mice, n = 8. Scale bars, 50μm. (H) Immunofluorescence staining to detect the proliferating cell marker Ki67 and quantification of Ki67 + cells in the epidermis of IMQ-induced WT or Postn −/− mice, n = 8. Scale bars, 50 μm. Quantitative data in (C, D, F, G, and H) are presented as mean ± SEM. ns , not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Two-way ANOVA, mixed-effects analysis with Tukey’s multiple comparisons test in (C, D, and F). Two-tailed unpaired Student’s t test in (G and H).

Article Snippet: For recombinant periostin stimulation, 1 μg/mL recombinant POSTN (rPOSTN; ABclonal) was added to the culture medium, which was refreshed daily throughout the experiment.

Techniques: Generated, Staining, Immunofluorescence, Marker, Two Tailed Test

Journal: iScience

Article Title: Periostin drives psoriasis progression through the stimulation of cutaneous nerve fibers

doi: 10.1016/j.isci.2026.116021

Figure Lengend Snippet: Reduced peripheral sensory neuron activation in Postn −/− mice (A, C and E) Immunofluorescent staining and quantification of TRPV1, CGRP and Nav1.8 positive neurons, n = 6. (B, D and F) Quantification of TRPV1, CGRP and Nav1.8 positive neurons. (G–J) RT-qPCR analysis of expression levels of markers associated with peripheral nociceptor receptors. Scale bars, 75 μm. Quantitative data in (B, D, F, G-J) are presented as mean ± SEM. ns , not significant, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. Two-way ANOVA, mixed-effects analysis with Tukey’s multiple comparisons test in (B, D and F, G-J).

Article Snippet: For recombinant periostin stimulation, 1 μg/mL recombinant POSTN (rPOSTN; ABclonal) was added to the culture medium, which was refreshed daily throughout the experiment.

Techniques: Activation Assay, Staining, Quantitative RT-PCR, Expressing

Journal: iScience

Article Title: Periostin drives psoriasis progression through the stimulation of cutaneous nerve fibers

doi: 10.1016/j.isci.2026.116021

Figure Lengend Snippet: Periostin regulates DRG axonal growth via the PI3K-AKT-mTOR signaling pathway (A) Schematic of the experimental workflow. (B) Volcano plot represents the differentially expressed genes between Postn −/− -vehicle and WT-vehicle groups. Genes that differed significantly compared to the WT-vehicle group, were colored with red (highly expressed in Postn −/− -Vehicle group) or blue (highly expressed in the WT-vehicle group). (C) Pathway Enrichment of Postn −/− -Vehicle group significant gene. (D and E) GSEA maps of the major differential gene sets in the Postn −/− -Vehicle and WT-Vehicle groups. (F) Immunoblot analysis of the PI3K/AKT pathway in WT-Vehicle and Postn −/− -Vehicle groups, n = 3. (G) PI3K, p-PI3K, AKT, and p -AKT protein levels were quantified, n = 3. (H) Volcano plot represents the differentially expressed genes between Postn −/− -IMQ and WT-IMQ groups. Genes that were differed significantly compared to the WT-IMQ group were colored in red (highly expressed in the Postn −/− -IMQ group) or blue (highly expressed in the WT-IMQ group). (I) GSEA maps of the major differential gene sets in the Postn −/− -IMQ and WT-IMQ groups. (J) Immunoblot analysis of the mTOR pathway in WT-IMQ and Postn −/− -IMQ groups, n = 3. (K) mTOR, p -mTOR protein levels were quantified, n = 3. Quantitative data in (G and K) are presented as mean ± SEM. ns , not significant, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001. Two-tailed unpaired Student’s t test in (G) and (K).

Article Snippet: For recombinant periostin stimulation, 1 μg/mL recombinant POSTN (rPOSTN; ABclonal) was added to the culture medium, which was refreshed daily throughout the experiment.

Techniques: Western Blot, Two Tailed Test

Journal: iScience

Article Title: Periostin drives psoriasis progression through the stimulation of cutaneous nerve fibers

doi: 10.1016/j.isci.2026.116021

Figure Lengend Snippet:

Article Snippet: For recombinant periostin stimulation, 1 μg/mL recombinant POSTN (rPOSTN; ABclonal) was added to the culture medium, which was refreshed daily throughout the experiment.

Techniques: Recombinant, Extraction, H&E Stain, Expressing, Next-Generation Sequencing, Single Cell, Software, Sequencing